This proposition describes a technique that allowed us to successfully stain human tissues with fluorochrome labelled antibodies for confocal and two-photon microscopy. Tissues are cut using either a microtome or a vibratome, fixed in parapholmaldeide and permebilized. This techinique is relatively complex and does not work for all the antibodies tested, the know-how to optimize the staining is relevant. The method can be of interest for partners interested in multi-parameter morphological analysis of human tissues.