Highly differentiated human hepatocytes in primary culture

Hepatocytes are isolated from human liver and cultured in a system that allows to maintain a highly differentiated phenotype for several weeks.rnIn these conditions, hepatocytes are sensitive to hepatitis C virus infection and permissive to viral genome replication.

Interest / Relevance: These cultures can be used to investigate: drug metabolism, drug toxicity, drug side effects, drug-mediate induction or inhibition of metabolism.rnThese cultures can be used as well for the screening of hepatitis C virus inhibitors.rnMore generally, these cultures can be used for investigating any aspect of liver physiopathology.

Keywords: hepatocyte, drug metabolism, HCV

Scientist's name: Patrick MAUREL
Lydiane PICHARD,Cédric DURET,Sabine CHALOIN GERBAL,Martine DAUJAT

Publications:

Pichard L, Raulet E, Fabre G, Ferrini JB, Ourlin JC, Maurel P. Human hepatocyte culture. Methods Mol Biol. 2006;320:283-93.rnrnDuret C, Daujat-Chavanieu M, Pascussi JM, Pichard-Garcia L, Balaguer P, Fabre JM, Vilarem MJ, Maurel P, Gerbal-Chaloin S. Ketoconazole and miconazole are antagonists of the human glucocorticoid receptor: consequences on the expression and function of the constitutive androstane receptor and the pregnane X receptor. Mol Pharmacol. 2006 Jul;70(1):329-39.rnrnMolina S, Castet V, Pichard-Garcia L, Wychowski C, Meurs E, Pascussi JM, Sureau C, Fabre JM, SaCunha A, Larrey D, Dubuisson J, Coste J, McKeating J, Maurel P, Fournier-Wirth C. Serum derived HCV infection of primary human hepatocytes is tetraspanin CD81 dependent. J Virol 2008 Jan;82(1):569-74.

Reference:

RT00321

Type of research tool: Know How

Therapy area(1): Oncology

Uses(1): Academic Research

Uses(2): Industry Research (screening, tox.studies, bioreactor, ...)

Uses(3): Diagnostic

Uses(4): Therapeutic

Inserm Transfert
Research Tools

E-mail

Accessible for: Fee-for-service research, Collaboration, Training / Know-How License

Equipment available: null

Samples/Models available: null

Rare disease: No

Last update: 12/06/2024

Highly differentiated human hepatocytes in primary culture

You might also be interested in

Selective antiestrogen binding site (aebs) ligands

Test rt 1707 v2

Transgenic mouse model for myotonic dystrophy:

Mouse anti-human cd11b (clone fn2h3)

Mouse anti-human flt3 (fms-like tyrosine kinase 3)

Anti-kiaa0753 rat monoclonal antibody 5h3

Mouse anti-human cd32 (clone 3d3)

Mouse anti-human follicular dendritic cells (clone

Human fcgriia/b (cd32 clone 6c4)

Rat anti-mouse cd8 beta subunit (mouse cd8beta)

Mouse anti-human cd1c (clone l161)

Mouse anti-human cd245 (clone dy12)