Back to results

Ex vivo culture system for screening compounds targeting leukaemia-initiating cells

A novel, ex vivo niche-based hypoxic culture system for maintaining Leukaemia Initiating Cells (LICs) from patients and an associated method to quantify their dynamics.

Interest / Relevance: The system and method permit efficient screening of compounds targeting leukaemia-initiating cells more quickly and affordable than the xenotransplantation assay in immunodeficient NSG mice. The approach also allows the analysis of effects/toxicity of compounds on normal hematopoïetic stem cells.

Keywords: Leukaemia Initiating Cells (LICs) , Acute Myeloid Leukaemia (AML) , Stem cells Niche , Self-renewal

Scientist's name: Emmanuel GRIESSINGER
Jean-François PEYRON

Publications:

Griessinger E et al. 2014. A Niche-Like Culture System Allowing the Maintenance of Primary Human Acute Myeloid Leukemia-Initiating Cells: A New Tool to Decipher Their Chemoresistance and Self-Renewal Mechanisms. Stem cells Translational Medicine. 3 520-9.

Reference:

RT00483

Type of research tool: Know How

Uses(1): Industry Research (screening, tox.studies, bioreactor, ...)

Inserm Transfert
Research Tools

Accessible for: Fee-for-service research, Collaboration, Training / Know-How License

Equipment available: This method requires a flow cytometer with at least two, at best three lasers, an hypoxia chamber or incubator and a Class II biosafety cabinet for handling human primary samples.

Samples/Models available: null

Rare disease: No

Last update: 12/06/2024

You might also be interested in

RT00439

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Musculoskeletal

Type of research tool: Know How

Real time no measurement

Real time constant amperometric detection of NO in tissues/cells.rnrnThis gaseous neurotransmitter […]

RT00396

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Others

Type of research tool: Know How

Three-dimensional multicellular spheroid cultures

When culture in suspension many mammalian cell types can aggregate into 3D-spheroids. These […]

RT00436

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Others

Type of research tool: Know How

Profiling blood lymphocytes reactivity against

In vitro trogocytosis of PKH67-stained cancer cells as a measure of their interactions with bulks of […]

RT00321

Uses: Therapeutic

Therapy Area: Oncology

Type of research tool: Know How

Highly differentiated human hepatocytes in primary

Hepatocytes are isolated from human liver and cultured in a system that allows to maintain a highly […]

RT00445

Uses: Theranostic (patients stratification, ...)

Therapy Area: Oncology

Type of research tool: Know How

Staining of human tissues for multi-color confocal

This proposition describes a technique that allowed us to successfully stain human tissues with […]

RT00488

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Genito Urinary System

Type of research tool: Know How

Technique to isolate live renal proximal tubular

We isolate renal proximal tubular cells using the proximal tubular marker Prominin-1.rnWe use a […]

RT00507

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Others

Type of research tool: Know How

3d cell omics – clinical proteomics platform

For your Clinical investigations, they propose to apply 3D culture cell methods to further your […]

RT00450

Uses: Diagnostic

Therapy Area: Genito Urinary System

Type of research tool: Know How

Urinary peptidome analysis

In collaboration with Mosaiques Diagnostics we can analyse biofluid proteomes for your needs using […]

RT00449

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Others

Type of research tool: Know How

Animal models of fibrosis

UUO: This model is induced in mice or rats by unilateral ureteral ligation (UUO) of the left ureter. […]

RT00422

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Oncology

Type of research tool: Know How

Screening and characterization of new histone

Using BRET technology, we designed a method to measure histone acetylation in living cells. Cells […]

RT00495

Uses: Therapeutic

Therapy Area: Oncology

Type of research tool: Know How

Establishment of redifferentiated cells after

When undifferentiated, invasive neoplastic cell lines are treated with an optimal time / […]

RT00453

Uses: Therapeutic

Therapy Area: Others

Type of research tool: Know How

Xenograft models to study stem and chemoresistant

We have recently contributed to establish a robust xenotransplantation model (NSG, NRG) to study the […]