Back to results

Three-dimensional multicellular spheroid cultures

When culture in suspension many mammalian cell types can aggregate into 3D-spheroids. These multicellular cultures mimic real tissues or tumours and are useful models for therapeutic screening.

Interest / Relevance: Intermediate assay between in cellulo and in vivo cultures; in some extent predictive for in vivo results.rnTest useful for:rn- Proliferation assaysrn- Cell-cell interaction analysisrn- Drug availability (clearance, half-life, ...)rn- Gene profilingrnrnAvantages: long term cultures, daily tracing of spheroid size.

Keywords: proliferation , tumour , cell toxicity , 3d-cultures , cell-cell interactions

Scientist's name: Annie MOLLA

Publications:

Hoang TM Favier B Valette A Barette C Nguyen CH Lafanechère L Grierson DS Dimitrov S Molla A.rnBenzo[e]pyridoindoles novel inhibitors of the aurora kinases.rnCell Cycle. 2009 Mar 1;8(5):765-72.

Reference:

RT00396

Type of research tool: Know How

Therapy area(1): Others

Uses(1): Academic Research

Uses(2): Proof of concept in vitro

Uses(3): Industry Research (screening, tox.studies, bioreactor, ...)

Inserm Transfert
Research Tools

Accessible for: Fee-for-service research, Collaboration, Training / Know-How License

Equipment available: Cell culture facilities/ microscopy

Samples/Models available: Many cell types are eligible.Requirement: aggregating cells.Examples: NSCLC H358, HCT-116, ...

Rare disease: No

Last update: 12/06/2024

You might also be interested in

RT00439

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Musculoskeletal

Type of research tool: Know How

Real time no measurement

Real time constant amperometric detection of NO in tissues/cells.rnrnThis gaseous neurotransmitter […]

RT00436

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Others

Type of research tool: Know How

Profiling blood lymphocytes reactivity against

In vitro trogocytosis of PKH67-stained cancer cells as a measure of their interactions with bulks of […]

RT00321

Uses: Therapeutic

Therapy Area: Oncology

Type of research tool: Know How

Highly differentiated human hepatocytes in primary

Hepatocytes are isolated from human liver and cultured in a system that allows to maintain a highly […]

RT00483

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Oncology

Type of research tool: Know How

Ex vivo culture system for screening compounds

A novel, ex vivo niche-based hypoxic culture system for maintaining Leukaemia Initiating Cells […]

RT00445

Uses: Theranostic (patients stratification, ...)

Therapy Area: Oncology

Type of research tool: Know How

Staining of human tissues for multi-color confocal

This proposition describes a technique that allowed us to successfully stain human tissues with […]

RT00488

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Genito Urinary System

Type of research tool: Know How

Technique to isolate live renal proximal tubular

We isolate renal proximal tubular cells using the proximal tubular marker Prominin-1.rnWe use a […]

RT00507

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Others

Type of research tool: Know How

3d cell omics – clinical proteomics platform

For your Clinical investigations, they propose to apply 3D culture cell methods to further your […]

RT00450

Uses: Diagnostic

Therapy Area: Genito Urinary System

Type of research tool: Know How

Urinary peptidome analysis

In collaboration with Mosaiques Diagnostics we can analyse biofluid proteomes for your needs using […]

RT00449

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Others

Type of research tool: Know How

Animal models of fibrosis

UUO: This model is induced in mice or rats by unilateral ureteral ligation (UUO) of the left ureter. […]

RT00422

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Oncology

Type of research tool: Know How

Screening and characterization of new histone

Using BRET technology, we designed a method to measure histone acetylation in living cells. Cells […]

RT00495

Uses: Therapeutic

Therapy Area: Oncology

Type of research tool: Know How

Establishment of redifferentiated cells after

When undifferentiated, invasive neoplastic cell lines are treated with an optimal time / […]

RT00453

Uses: Therapeutic

Therapy Area: Others

Type of research tool: Know How

Xenograft models to study stem and chemoresistant

We have recently contributed to establish a robust xenotransplantation model (NSG, NRG) to study the […]