Highly differentiated human hepatocytes in primary culture

Hepatocytes are isolated from human liver and cultured in a system that allows to maintain a highly differentiated phenotype for several weeks.rnIn these conditions, hepatocytes are sensitive to hepatitis C virus infection and permissive to viral genome replication.

Interest / Relevance: These cultures can be used to investigate: drug metabolism, drug toxicity, drug side effects, drug-mediate induction or inhibition of metabolism.rnThese cultures can be used as well for the screening of hepatitis C virus inhibitors.rnMore generally, these cultures can be used for investigating any aspect of liver physiopathology.

Keywords: hepatocyte, drug metabolism, HCV

Scientist's name: Patrick MAUREL
Lydiane PICHARD,Cédric DURET,Sabine CHALOIN GERBAL,Martine DAUJAT

Publications:

Pichard L Raulet E Fabre G Ferrini JB Ourlin JC Maurel P. Human hepatocyte culture. Methods Mol Biol. 2006;320:283-93.rnrnDuret C Daujat-Chavanieu M Pascussi JM Pichard-Garcia L Balaguer P Fabre JM Vilarem MJ Maurel P Gerbal-Chaloin S. Ketoconazole and miconazole are antagonists of the human glucocorticoid receptor: consequences on the expression and function of the constitutive androstane receptor and the pregnane X receptor. Mol Pharmacol. 2006 Jul;70(1):329-39.rnrnMolina S Castet V Pichard-Garcia L Wychowski C Meurs E Pascussi JM Sureau C Fabre JM SaCunha A Larrey D Dubuisson J Coste J McKeating J Maurel P Fournier-Wirth C. Serum derived HCV infection of primary human hepatocytes is tetraspanin CD81 dependent. J Virol 2008 Jan;82(1):569-74.

Reference:

RT00321