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Technique to isolate live renal proximal tubular cells from any mouse strain

We isolate renal proximal tubular cells using the proximal tubular marker Prominin-1.rnWe use a 4°C, enzyme free protocole allowing high quality subsequent analyses (e.g. transcriptome), and obtention of primary cultures.

Interest / Relevance: This allows to study specifically a major cell type of the kidney during in vivo experiments, and to obtain well-differentiated primary proximal tubular cell cultures of any mouse strain for in vitro experiments.

Keywords: proximal tubule , renal primary culture , ex vivo

Scientist's name: Pierre GALICHON
Sophie Vandermeersch

Publications:

Legouis D Bataille A Hertig A Vandermeersch S Simon N Rondeau E Galichon P (2015) Ex vivo analysis of renal proximal tubular cells. BMC Cell Biology 16(1):12.

Reference:

RT00488

Type of research tool: Know How

Uses(1): Academic Research

Uses(2): Proof of concept in vitro

Uses(3): Proof of concept in vivo

Uses(4): Industry Research (screening, tox.studies, bioreactor, ...)

Antibody applications: In vivo studies

Inserm Transfert
Research Tools

Accessible for: Fee-for-service research, Collaboration, Training / Know-How License

Equipment available: Freshly isolated mouse proximal tubular cells.Primary cell cultures, Protein and mRNA samples.

Samples/Models available: Ischemia repercussion injury (in vivo and in vitro)In vivo and in vitro toxicity of cyclosporine (and other toxicants)

Rare disease: No

Last update: 12/06/2024

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