Vhh directed against human darc

VHH (i.e. recombinant derivative of peculiar heavy chain only antibodies present in camelids) directed against Duffy antigen receptor for chemokines (DARC). DARC which carries antigens defining the Duffy blood group system, is a human atypical chemokine receptor, the major receptor for Plasmodium vivax merozoite on erythrocytes, a protein implicated in cancer, inflammation, possibly HIV infection and AIDS progression.VHH was obtained afer screening of a library prepared from a dromedary immunized against first extracellular domain of DARC. VHH does recognize DARC on cells and interacts with the Fy6 epitope. It does inhibit P. vivax invasion and chemokine binding (IL8). Its affinity to DARC is in the subnanomolar range. Expression plasmid (6 His) codes for a hemagglutinin tagged VHH.

Interest / Relevance: Immunological tool to study DARC
Keywords: VHH , recombinant antibody fragment , sdAb , DARC
Scientist's name: Yves COLIN-ARONOVICZ
Caroline LE VAN KIM,Dorota SMOLAREK
Publications:
Smolarek D Hattab C Hassanzadeh-Ghassabeh G Cochet S Gutiérrez C de Brevern AG Udomsangpetch R Picot J Grodecka M Wasniowska K Muyldermans S Colin Y Le Van Kim C Czerwinski M Bertrand O. A recombinant dromedary antibody fragment (VHH or nanobody) directed against human Duffy antigen receptor for chemokines. Cell Mol Life Sci. 2010 Oct;67(19):3371-87.

Reference:

RT00459

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Clones available: Expression plasmids
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Isotype: null
Dromedary
Source: Dromedary
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Specificity: null
Immunogen Seq ID: null
Cellular Distribution: null
Know Cross Reactivity: null
Depositor: null
FBS: null
Rare disease: No
Last update: 18/06/2024

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