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Primary cultures of mice proximal tubular cells

Proximal tubular cells are isolated by magnetic sorting coupled with microbeads-conjugated anti-Prominin-1 antibodies. Prominin-1 colocalizes with Megalin in normal and pathological kidneys.rnrnProximal tubular cells are well-differentiated and keep a proliferative potential after dozens of passages in culture.

Interest / Relevance: Good model for in vitro experiments on the proximal tubule, with the possibility of obtaining primary cell cultures from any mouse strain, including genetically modified animals (upon request).rnproximal tubular injury (schema-reperfusion, toxicants..)

Keywords: Proximal tubule , Primary culture

Scientist's name: Pierre GALICHON
Sophie Vandermeersch

Publications:

Legouis D Bataille A Hertig A Vandermeersch S Simon N Rondeau E Galichon P (2015) Ex vivo analysis of renal proximal tubular cells. BMC Cell Biology 16(1):12

Reference:

RT00486

Type of research tool: Cell Lines

Uses(1): Academic Research

Uses(2): Proof of concept in vitro

Uses(3): Industry Research (screening, tox.studies, bioreactor, ...)

Inserm Transfert
Research Tools

Organism: Mus musculus

Tissue: Kidney

Morphology: null

Passage Number: 1-30

Subculture Routine: null

Culture Medium: The composition or the medium itself is provided with the cells.

Growth Properties: Proliferative potential remains after 30 passages

Karyotype: null

Rare disease: No

Last update: 12/06/2024

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