HG5LN were generated by transfection of cervical cancer HeLa cells with the (GAL4RE)5-betaGlobin-Luciferase-SV40-Neomycin plasmid. rnHG5LN GAL4-hPPAR beta/delta cells were generated by transfection of HG5LN cells with the plasmid pSG5-GAL4-human PPAR beta/delta-puromycin.
The plasmid (GAL4RE)5-betaGlob-Luc-SVNeo contains a luciferase gene driven by a pentamer of yeast activator GAL4 binding sites in front of beta-globin promoter and a neomycin phosphotransferase gene under the control of SV40 promoter. rnThe pSG5-puro plasmid pSG5-human GAL4-hPPAR beta/delta -puromycin enables to express the DNA binding domain of the yeast activator GAL4 (GAL4 DBD) followed by the ligand binding domain of the human peroxisome proliferator activated receptor receptor beta/delta (hPPAR beta/delta LBD). Puromycin N-acetyl transferase selection marker expression confers resistance to puromycin.rn