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Ms-5-omi/htra2-mcherry cells

Murine stromal MS-5 cells were transduced with a retroviral pBabe vector to stably express the Omi/HTRA2-mCherry protein. rnOMI/HTRA2 is a marker of intact polarized mitochondria that is rapidly degraded following mitochondrial outer membrane permeabilization (MOMP).

Interest / Relevance: Feeder cells for long-term culture of normal and Acute Leukemia Myeloid cellsrnmCherry fluorescence is a read-out of mitochondria integrity. rnMS5-Omi/HTRA2-mCherry cells can be used to study mitochondrial transfer.rnMouse Stromal cells

Keywords: Stromal cells , LTC-IC , Omi-mCherry , Mitochondrial transfer

Scientist's name: Véronique IMBERT
Jean-François PEYRON

Publications:

Moschoi R. Imbert V. Nebout M. Chiche J. Mary D. Prebet T. Saland E. Castellano R. Pouyet L. Collette Y. Vey N. Chabannon C. Recher C. Sarry J. E. Alcor D. Peyron J. F. and Griessinger E. (2016). Protective mitochondrial transfer from bone marrow stromal cells to acute myeloid leukemic cells during chemotherapy. Blood 128 253-264.

Reference:

RT00494

Type of research tool: Cell Lines

Therapy area(1): Others

Uses(1): Academic Research

Uses(2): Proof of concept in vitro

Uses(3): Industry Research (screening, tox.studies, bioreactor, ...)

Inserm Transfert
Research Tools

Organism: Mouse

Tissue: Stromal cells

Morphology: Adherent cells

Passage Number: null

Subculture Routine: Split confluent culture 1:3 to 1:10 every third day using trypsin/EDTA

Culture Medium: IMDM supplemented with 2mM L-Glutamine 25mM Hepes 10%SVF Penicillin Streptomycin

Growth Properties: null

Karyotype: null

Rare disease: No

Last update: 12/06/2024

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