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Mouse t lymphoma cell line expressing luciferase – ko99l/luc

The primary KO99L cell line was established by cultivating tumoral thymocytes from a tPTEN-/- mouse. KO99L cells were transduced with a pLenti-III-luc23 construct to stably express luciferase. rnSeveral clones are available.

Interest / Relevance: This cell line is suitale for in vitro and in vivo analyses of tumor growth by bioluminescence. rnTumorigenic in nude mice.

Keywords: T ALL/LL , Imaging , Tumor growth , Bioluminescence

Scientist's name: Véronique IMBERT
Jean-François PEYRON

Publications:

Rosilio C. Lounnas N. Nebout M. Imbert V. Hagenbeek T. Spits H. Asnafi V. Pontier-Bres R. Reverso J. Michiels J. F. Sahra I. B. Bost F. and Peyron J. F. (2013). The metabolic perturbators metformin phenformin and AICAR interfere with the growth and survival of murine PTEN-deficient T cell lymphomas and human T-ALL/T-LL cancer cells. Cancer Lett 336 114-126.rnrnrnRosilio C. Nebout M. Imbert V. Griessinger E. Neffati Z. Benadiba J. Hagenbeek T. Spits H. Reverso J. Ambrosetti D. Michiels J. F. Bailly-Maitre B. Endou H. Wempe M. F. and Peyron J. F. (2015). L-type amino-acid transporter 1 (LAT1): a therapeutic target supporting growth and survival of T-cell lymphoblastic lymphoma/T-cell acute lymphoblastic leukemia. Leukemia 29 1253-1266.

Reference:

RT00492

Type of research tool: Cell Lines

Therapy area(1): Others

Uses(1): Academic Research

Uses(2): Proof of concept in vitro

Uses(3): Proof of concept in vivo

Uses(4): Industry Research (screening, tox.studies, bioreactor, ...)

Antibody applications: In vivo studies

Inserm Transfert
Research Tools

Organism: Mouse

Tissue: Thymocytes, T lymphoma

Morphology: Suspension, round cells

Passage Number: null

Subculture Routine: Medium Renewal: Every 2 to 3 days Dilution 1:10

Culture Medium: RPMI 1640 Glutamax supplemented with 20% FCS, 50 U/ml penicillin, 50 µg/ml streptomycin, and 1 mM sodium pyruvate.

Growth Properties: null

Karyotype: null

Rare disease: No

Last update: 12/06/2024

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