Mouse model foxp3-egfp

The targeting vector was designed to insert (from 5' to 3') an internal ribosomal entry site (IRES) fused to an enhanced green fluorescent protein (EGFP) and a self-excising loxP-flanked ACN cassette into exon 11 of the X-linked forkhead box P3 (Foxp3) gene. The ACN cassette contained a neomycin resistance (Neo) gene and a testes-specific angiotensin-converting enzyme promoter-driven Cre recombinase gene (tACE-Cre). The construct was electroporated into C57BL/6-derived Bruce-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into FVB/N blastocysts and chimeric males were bred to C57BL/6J females. The resulting Foxp3GFP mice were maintained on a C57BL/6J background. This reporter mouse model is deposited at The Jackson Laboratory (Jax stock#018628).

Interest / Relevance: This model is a reporter mouse model.
This model targets the IRES-GFP cassette to exon 11, downstream of the endogenous Foxp3 STOP codon with no defects in FOXP3 or regulatory T cell function observed. EGFP is expressed from the forkhead box P3 (Foxp3) locus in these mice. Foxp3 expression is not disrupted. Foxp3+ Treg cells can be identified in these mice by EGFP expression.
Keywords: FoxP3
Publications:
J Immunol 2008 180(3):1565-75 Eur J Immunol. 2012 Dec;42(12):3150-66. doi: 10.1002/eji.201242847. Epub 2012 Oct 17. J Immunol. 2013 Apr 1;190(7):3235-45. doi: 10.4049/jimmunol.1201396. Epub 2013 Mar 1.

Reference:

RT00482

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Bernard MALISSEN
Inserm Transfert
Research Tools
Species: Mouse
Strain: B6.Cg-Thy1
Genetic Background: C57BL/6J
Applications: Immunology
Rare disease: No
Last update: 10/10/2022

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