Back to results

Hrln (hrar alpha) cell line

HRLN were generated by transfection of cervical cancer HeLa cells with the (RARE)3-TK-Luciferase-SV40-Neomycin plasmid. rnThe plasmid (RARE)3-TK-Luc-SVNeo contains a luciferase gene driven by three retinoid receptor responsive element (RARE) in front of thymidine kinase promoter and a neomycin phosphotransferase gene under the control of SV40 promoter. rn

Interest / Relevance: These cells enable to measure the hRAR alpha activity (full and partial agonism, antagonism) of pharmaceuticals (breast cancer, menopause treatment), environmental compounds (alkylphenols, bisphenol A, phthalates or pesticides, cosmetics). rnThese cells enable to measure the hRAR alpha activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.rnrnTTNPB EC50 0.8 nMrn

Keywords: hRAR alpha, luciferase, natural, pharmaceutical and environmental retinoids

Scientist's name: Patrick BALAGUER
Patrick Balaguer

Publications:

Balaguer P Boussioux AM Demirpence E Nicolas JC. Reporter cell lines are useful tools for monitoring biological activity of nuclear receptor ligands. Luminescence. 2001 16:1 53-8.

Reference:

RT00623

Type of research tool: Cell Lines

Uses(1): Industry Research (screening, tox.studies, bioreactor, ...)

Inserm Transfert
Research Tools

Organism: null

Tissue: null

Morphology: null

Passage Number: null

Subculture Routine: null

Culture Medium: null

Growth Properties: null

Karyotype: null

Rare disease: No

Last update: 12/06/2024

You might also be interested in

RT00625

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Oncology

Type of research tool: Cell Lines

Ug5ln cell line

UG5LN were generated by transfection of osteocarcinoma cancer U20S cells with the […]

RT00624

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Oncology

Type of research tool: Cell Lines

Ualh cell line

UALH were generated by transfection of osteocarcinoma cancer U2OS cells with the […]

RT00586

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Oncology

Type of research tool: Cell Lines

Ualh har cell line

UALH were generated by transfection of osteocarcinoma cancer U2OS cells with the […]

RT00582

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Oncology

Type of research tool: Cell Lines

Palm cell line

PALM were generated by co-transfection of cervical cancer PC3 cells with the […]

RT00583

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Oncology

Type of research tool: Cell Lines

Meln (her alpha) cell line

MELN were generated by transfection of breasr cancer MCF-7 cells with the […]

RT00626

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Oncology

Type of research tool: Cell Lines

Ug5ln gal4-hmr cell line

UG5LN were generated by transfection of osteocarcinoma cancer U2OS cells with the […]

RT00622

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Oncology

Type of research tool: Cell Lines

Hmln (hgr) reporter cell line

HMLN were generated by transfection of cervical cancer HeLa cells with the MMTV- […]

RT00558

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Oncology

Type of research tool: Cell Lines

Hg5ln gal4-hppar alpha cell line

HG5LN were generated by transfection of cervical cancer HeLa cells with the […]

RT00559

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Oncology

Type of research tool: Cell Lines

Hg5ln gal4-hppar gamma cell line

HG5LN were generated by transfection of cervical cancer HeLa cells with the […]

RT00569

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Oncology

Type of research tool: Cell Lines

Hg5ln gal4-hppar beta/delta cell line

HG5LN were generated by transfection of cervical cancer HeLa cells with the […]

RT00585

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Oncology

Type of research tool: Cell Lines

Hg5ln rtr alpha cell line

HG5LN were generated by transfection of cervical cancer HeLa cells with the […]

RT00588

Uses: Industry Research (screening, tox.studies, bioreactor, ...)

Therapy Area: Oncology

Type of research tool: Cell Lines

Hg5ln hpxr cell line

HG5LN were generated by transfection of cervical cancer HeLa cells with the […]