Cpt1 expressing yeast strains

Saccharomyces cerevisiae expressing under the GAL promoter either the wild-type rat liver CPT1, mutated rat liver CPT1 or chimeric CPT. CPT1A activity is inhibited by malonyl-CoA, its allosteric inhibitor.

Interest / Relevance: Allow to have isolated mitochondria with wild-type rat liver CPT1 (active and sensitive to malonyl-CoA inhibition) or mutated CPT1 (for example, active but insensitive to malonyl-CoA inhibition). These mitochondria could be used as biological material for screening chemical molecules able to modulate CPT1 activity and malonyl-CoA sensitivity.
Scientist's name: Carina PRIP-BUUS



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Inserm Transfert
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Organism: Saccharomyces cerevisiae
Tissue: Unicellular
Morphology: Not relevant
Passage Number: Not relevant
Subculture Routine: Not relevant
Culture Medium: Minimal selective medium. Induction of protein expression by addition of galactose
Growth Properties: Not relevant
Karyotype: Not relevant
About pre-identified partners : if you are already in contact with one or several potential partner(s), please provide the list of such partner(s) and as much detail as possible: No
Rare disease: No
Last update: 15/12/2021

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