Cardiac overexpression of the human beta3-adrenergic receptor.

Mice with a cardiac ovexpression of the human beta3-adrenergic receptor were produced by microinjection of a construct with A or C isoform gene under the control of the alpha myosin heavy chain promoter. Phenotypic analyses comprised beta3-AR mRNA and protein determinations, histological studies, electrocardiogram, contractility and cyclic nucleotide measurements. Transgenic mice that ovexepressed the A form were cryopreserved while transgenic mice that ovexepressed the C form are already alive.

Interest / Relevance: Pathway analysis from the receptor activation to the physiological effect.
Screening of new compounds acting on human beta3-AR prior to clinical studies.
Keywords: adrenergic , heart , secreening
beta3-Adrenergic stimulation produces a decrease of cardiac contractility ex vivo in mice overexpressing the human beta3-adrenergic receptor. Tavernier G Toumaniantz G Erfanian M Heymann MF Laurent K Langin D Gauthier C. Cardiovasc Res. 2003 Aug 1;59(2):288-96. Interspecies differences in the cardiac negative inotropic effects of beta(3)-adrenoceptor agonists. Gauthier C Tavernier G Trochu JN Leblais V Laurent K Langin D Escande D Le Marec H. J Pharmacol Exp Ther. 1999 Aug;290(2):687-93.



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Inserm Transfert
Research Tools
Species: Mus musculus
Genotype: Hemizygous
Strain: alphaMHC-hu beta3AR (MHC : myosin heavy chain)
Genetic Background: B6D2
Applications: beta3-adrenergic agonist screening, cardiac parameters
Rare disease:
Last update: 15/12/2021

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