Ug5ln gal4-hmr cell line

UG5LN were generated by transfection of osteocarcinoma cancer U2OS cells with the (GAL4RE)5-betaGlobin-Luciferase-SV40-Neomycin plasmid.rnUG5LN GAL4-hMR cells were generated by transfection of UG5LN cells with the pSG5-puro plasmid pSG5-GAL4-human MR-puromycin.rnThe plasmid (GAL4RE)5-betaGlob-Luc-SVNeo contains a luciferase gene driven by a pentamer of yeast activator GAL4 binding sites in front of beta-globin promoter and a neomycin phosphotransferase gene under the control of SV40 promoter.rnThe pSG5-puro plasmid pSG5-human GAL4-hMR-puromycin enables to express the DNA binding domain of the yeast activator GAL4 (GAL4 DBD) followed by the ligand binding domain of the human mineralocorticoid receptor (hMR LBD). Puromycin N-acetyl transferase selection marker expression confers resistance to puromycin.

Interest / Relevance: These cells enable to measure the hMR (full and partial agonism, antagonism) of pharmaceuticals, environmental compoundsrnThese cells enable to measure the hMR activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.rnrnaldosterone EC50 0.6 nM
Keywords: hMR, luciferase, metabolism, clearance, colon and liver cancer, pharmaceutical and environmental chemicals
Scientist's name: Patrick BALAGUER



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Inserm Transfert
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Organism: null
Tissue: null
Morphology: null
Passage Number: null
Subculture Routine: null
Culture Medium: null
Growth Properties: null
Karyotype: null
Rare disease: No
Last update: 12/06/2024

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