Ht29-mtx cells : intestinal model of goblet cells

HT29MTX10-6 and HT29MTX10-5 cell populations were isolated from the colon cancer cell line by stepwise adaptation to 10-6 M and 10-5 M Methotrexate (MTX), respectively. HT29MTX cells form a homogeneous population of polarized goblet cells that secrete mucins of gastric immunoreactivity. A mucus gel becomes visible on the surface of the cell layer at postconfluency. These cells maintain their ability to differentiate under normal culture conditions (without MTX).

This cell line is available through CelluloNet in Lyon

Interest / Relevance: Polarized cellsrnMucus gel on the cell surfacernProvide tumor in nude mice (subcutaneous injection)rnGrowth on tissue culture treated plastic and Transwell membranernstudy on proteins linked to goblet cell phenotype, interactions with pathogens, drug transport, drug resistance, co-culture with enterocyte cells

Scientist's name: Thecla LESUFFLEUR

Publications:

- Lesuffleur et al. 1990 Cancer Research 50 : 6334-6343rn- Lesuffleur et al. 1991 J Cell Biol 115 : 1409-1418rn- Lesuffleur et al. 1993 J. Cell Science 106 : 771-783

Reference:

RT00309

Type of research tool: Cell Lines

Therapy area(1): Oncology

Uses(1): Academic Research

Uses(2): Industry Research (screening, tox.studies, bioreactor, ...)

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Organism: human

Tissue: colon tumor

Morphology: adherente, monolayer of polarized goblet cells with an apical brush border

Passage Number: 12 to 18

Subculture Routine: the cells are passaged every week using 0,05% Trypsin / EDTA and seeded at 2,4x104 cells per cm2

Culture Medium: DMEM Glutamax (4.5 g/L glucose) supplemented with 10% of inactivated FBS.The medium is changed daily from the second day after seeding on (5ml/25cm2)

Growth Properties: doubling-time : 28 hours

Karyotype: aneuploid

Rare disease: No

Last update: 12/06/2024

Ht29-mtx cells : intestinal model of goblet cells

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