Heln er beta cell line

HELN were generated by transfection of cervical cancer HeLa cells with the ERE-betaGlobin-Luciferase-SV40-Neomycin plasmid. rnHELN hER beta cells were generated by transfection of HELN cells with the plasmid pSG5-human ERbeta-puromycin. rnrnrnLignée cellulaire de cancer du col de l?utérus HeLa (ER négative) transfectée premièrement par le plasmide de la luciférase firefly sous le contrôle d?un élément de réponse au récepteur des oestrogènes ERE, secondairement par un plasmide d?expression du récepteur des oestrogènes humain beta

Interest / Relevance: These cells enable to measure the hER beta estrogenic activity (full and partial agonism, antagonism) of pharmaceuticals (breast cancer, menopause treatment), environmental compounds (alkylphenols, bisphenol A, phthalates or pesticides, cosmetics). rnThese cells enable to measure the hER beta estrogenic activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.rnEstradiol EC50 68 pM
Keywords: hER beta, luciferase, pharmaceutical and environmental estrogens
Scientist's name: Patrick BALAGUER
Patrick Balaguer
Publications:
Escande A Pillon A Servant N Cravedi JP Larrea F Muhn P Nicolas JC Cavailles V Balaguer P. Evaluation of ligand selectivity using reporter cell lines stably expressing estrogen receptor alpha or beta. Biochem Pharmacol. 2006 71: 1459-69.rnDelfosse V Grimaldi M Pons JL Boulahtouf A le Maire A Cavailles V Labesse G Bourguet W Balaguer P. Structural and mechanistic insights into bisphenols action provide guidelines for risk assessment and discovery of bisphenol A substitutes. Proc Natl Acad Sci U S A. 2012 109 : 14930-5.rnDelfosse V Grimaldi M Cavaillès V Balaguer P Bourguet W. Structural and Functional Profiling of Estrogen Receptors Environmental Ligands. Environ Health Perspect. 2014 122: 1306-13.rn

Reference:

RT00317

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Organism: human
Tissue: cervix
Morphology: null
Passage Number: null
Subculture Routine: null
Culture Medium: null
Growth Properties: null
Karyotype: null
Rare disease: No
Last update: 12/06/2024

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