Ualh har cell line

UALH were generated by transfection of osteocarcinoma cancer U2OS cells with the (ARE-RAD9)6-collagenase-Luciferase-SV40-hygromycin plasmid. rnUALH hAR cells were generated by co-transfection of UALH cells with the plasmids pSG5-hAR-puromycin and pSG5-hAR-Renilla-luciferase-(ARE-RAD9)6-collagenase-Luciferase-SV40-neomycin. rnThe plasmid (ARE RAD9)6-Coll-Luc-SVNeo contains a luciferase gene driven by the mouse mammary tumor virus promoter and a neomycin phosphotransferase gene under the control of SV40 promoter. rnThe pSG5-puro plasmid pSG5-human AR-puromycin enables to express the human androgen receptor (hAR). Puromycin N-acetyl transferase selection marker expression confers resistance to puromycin. rnThe plasmid pSG5-hAR-Renilla-Neomycin contains the gene coding for hAR under the control of the SV40 promoter, the Renilla luciferase reporter gene under the control of the constitutive human cytomegalovirus (CMV), the firefly luciferase gene under the control of six androgen response elements (ARE) from the RAD9 gene promoter and placed upstream of the collagenase promoter, and the resistance gene to neomycin under the control of the SV40 promoter. This third plasmid has been included to over-express both AR and luciferase expression.rn

Interest / Relevance: These cells enable to measure the hAR activity (full and partial agonism, antagonism) of pharmaceuticals (breast cancer, menopause treatment), environmental compounds (alkylphenols, bisphenol A, phthalates or pesticides, cosmetics). rnThese cells enable to measure the hAR activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.rnrnR1181 EC50 0,16 nMrn
Keywords: hAR, luciferase, pharmaceutical and environmental androgens
Scientist's name: Patrick BALAGUER
Boulahtouf Abdelhay, Grimaldi Marina
Publications:
Neale P Grimaldi M Boulahtouf A Leusch F Balaguer P. Assessing species-specific differences for nuclear receptor activation for environmental water extracts. Water Research 2020 185 : 116247.rnrnGaroche C Grimaldi M Boulahtouf A Michelin E Marconi A Brion F Balaguer P Aït-Aïssa S. Interlaboratory prevalidation of a new in vitro transcriptional activation assay for the screening of (anti-) androgenic chemicals using the U2OS-hAR2 cell line. TIV 2023. In Press.rn

Reference:

RT00586

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Organism: null
Tissue: null
Morphology: null
Passage Number: null
Subculture Routine: null
Culture Medium: null
Growth Properties: null
Karyotype: null
Rare disease: No
Last update: 12/06/2024

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