Stable human prolactin receptor ba/f3 cell

Ba/F3 cells are mouse pro-B lymphocytes that are dependent on IL-3 for survival/growth. We stably transfeted them with expression vector encoding the long isoform of the human prolactin receptor. Their survival/growth can now be shifted to prolactin in the absence of IL-3.

Interest / Relevance: It is used to test any ligand of the prolactin receptor, based on cell proliferation and survival. It can also be used for signaling studies of the prolactin receptor. It can also be usd for evaluating the amount of prolactin receptor agonist in a fluid.

Keywords: prolactin , prolactin receptor , luciferase , agonists , antagonists

Scientist's name: Vincent GOFFIN

Publications:

1.Bernichtein S et al (2003) Endocrine 20: 177-190.rn2.Bernichtein S et al (2003) J Biol Chem 278: 35988-35999.rn3.Glezer et al (2006) JCEM 91: 1048-1055.

Reference:

RT00003

Type of research tool: Cell Lines

Uses(1): Academic Research

Uses(2): Industry Research (screening, tox.studies, bioreactor, ...)

Inserm Transfert
Research Tools

E-mail

Organism: Mouse

Tissue: Pro-B lymphocytes

Morphology: Suspension, round cells

Passage Number: null

Subculture Routine: null

Culture Medium: Classical RPMI with additives

Growth Properties: Passage every other day (do not like being too diluted or confluent)

Karyotype: null

Rare disease: No

Last update: 12/06/2024

Stable human prolactin receptor ba/f3 cell

You might also be interested in

Test debug

Use of irap inhibitors for the treatment of

Safety and efficacy of mait cells for adoptive

Intestinal cathelicidin antimicrobial peptide

Use of hedgehog inhibitors for the treatment of

Methods for the diagnosis and thetreatment of

Fc-engineered anti-human ige antibodies and

Use of etv3 or etv6 inhibitors for blocking the

Methods for modulating innate lymphoid cell

Use of tcr-deficient car-tregs in combination with