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Rat mesothelioma cell line m5-t1

Neoplastic rat cell line isolated from tumor masses (peritoneal mesothelioma) collected in the peritoneal cavity of a male rat (F344) after 378 days of induction with crocidolite (blue asbestos) injected intraperitoneously.
Interest / Relevance: - Academic Research: identification of markers of neoplastic / transformed mesothelial cells, analyses of chromosomal damage induced by asbestos, studies of the mechanism of asbestos-induced oncogenesis.
- Industry research: antibody production, screening of new anticancer agents, identification of factors implicated in mesothelioma cell proliferation, migration, and invasion.
- Therapeutic: development of epigenetic drugs, design of cancer vaccines.
Keywords: mesothelial cells , tumor marker , tumor invasion process , malignant transformation , proliferation rate
Scientist's name: Daniel POULIQUEN

Reference:

RT00417

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Daniel POULIQUEN
Inserm Transfert
Research Tools
Organism: Rat
Tissue: Tumor (mesothelioma)
Morphology: Sarcomatoid, formation of spheroids in culture
Passage Number: 2, 5
Culture Medium: RPMI 1640 supplemented with 10% FCS and 2mM L-Glutamine
Growth Properties: In 6-well plates (300,000 cells/well): Doubling time <
24h. Saturation density >200,000 cells / sq-cm.
Karyotype: under investigation
Rare disease:
Last update: 08/02/2022

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