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Rat mesothelial cell line f3-1

Transformed rat mesothelial cell line isolated from the peritoneal fluid of a female rat (Fischer F344) after 356 days of induction with crocidolite (blue asbestos) injected intraperitoneously.

Interest / Relevance: - Academic Research: identification of markers of neoplastic / transformed mesothelial cells, analyses of chromosomal damage induced by asbestos, studies of the mechanism of asbestos-induced oncogenesis, implication of fibrosis and inflammation.rn- Industry research: antibody production, screening of new anticancer agents, identification of factors that increase mesothelial proliferation and migration. rn- Therapeutic: development of epigenetic drugs, design of cancer vaccines, regenerative medicine applications.rn rnCellular and Molecular Biology, Immunology.

Scientist's name: Daniel POULIQUEN

Publications:

null

Reference:

RT00415

Type of research tool: Cell Lines

Therapy area(1): Infectious Diseases

Therapy area(2): Oncology

Therapy area(3): Others

Uses(1): Academic Research

Uses(2): Proof of concept in vitro

Uses(3): Industry Research (screening, tox.studies, bioreactor, ...)

Uses(4): Diagnostic

Uses(5): Therapeutic

Inserm Transfert
Research Tools

Organism: Rat

Tissue: Mesothelium (peritoneal cavity)

Morphology: Epithelioid

Passage Number: 4

Subculture Routine: null

Culture Medium: RPMI 1640 supplemented with 10% FCS and 2mM L-Glutamine

Growth Properties: In 6-well plates (300,000 cells/well): Doubling time: 24-48h. Saturation density >200,000 cells / sq-cm.

Karyotype: under investigation

Rare disease: No

Last update: 12/06/2024

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