Rat mesothelial cell line f1-0

Transformed rat mesothelial cell line isolated from the peritoneal fluid of a female rat (Fischer F344) after 277 days of induction with crocidolite (blue asbestos) injected intraperitoneously.

Interest / Relevance: - Academic Research: identification of markers of neoplastic / transformed mesothelial cells, analyses of chromosomal damage induced by asbestos, studies of the mechanism of asbestos-induced oncogenesis, implication of fibrosis and inflammation.rn- Industry research: antibody production, screening of new anticancer agents, identification of factors that increase mesothelial proliferation and migration. rn- Therapeutic: development of epigenetic drugs, design of cancer vaccines, regenerative medicine applications.
Keywords: mesothelial cells , tumor marker , tumor invasion process , malignant transformation , proliferation rate
Scientist's name: Daniel POULIQUEN
Publications:
null

Reference:

RT00420

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Inserm Transfert
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Organism: Rat
Tissue: Mesothelium (peritoneal cavity)
Morphology: 2 subtypes, one epithelioid (F1-0Ep) and the second mixt, enriched with sarcomatoid cells (F1-0Fib)
Passage Number: F1-0Ep: passages 4 and 7
F1-0Fib: passage 6
Subculture Routine: null
Culture Medium: RPMI 1640 supplemented with 10% FCS and 2mM L-Glutamine
Growth Properties: In 6-well plates (300,000 cells/well): Doubling time 24-48h. Saturation density: 100,000-200,000 cells / sq-cm.
Karyotype: under investigation
Rare disease: No
Last update: 12/06/2024

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