Palm cell line

PALM were generated by co-transfection of cervical cancer PC3 cells with the MMTV-Luciferase-SV40-Neomycin and the pSG5-human AR-puromycin plasmids. rnThe plasmid MMTV-Luc-SVNeo contains a luciferase gene driven by the mouse mammary tumor virus promoter and a neomycin phosphotransferase gene under the control of SV40 promoter. rnThe pSG5-puro plasmid pSG5-human AR-puromycin enables to express the human androgen receptor (hAR). Puromycin N-acetyl transferase selection marker expression confers resistance to puromycin. rn

Interest / Relevance: These cells enable to measure the hAR activity (full and partial agonism, antagonism) of pharmaceuticals (breast cancer, menopause treatment), environmental compounds (alkylphenols, bisphenol A, phthalates or pesticides, cosmetics). rnThese cells enable to measure the hAR activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.rnrnR1881 EC50 0,05 nMrn
Keywords: hAR, luciferase, pharmaceutical and environmental androgens
Scientist's name: Patrick BALAGUER
Patrick Balaguer
Publications:
Terouanne B Tahiri B Georget V Belon C Poujol N Avances C Orio F Jr Balaguer P Sultan C. A stable prostatic bioluminescent cell line to investigate androgen and antiandrogen effects. Mol Cell Endocrinol. 2000 160: 39-49

Reference:

RT00582

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Organism: null
Tissue: null
Morphology: null
Passage Number: null
Subculture Routine: null
Culture Medium: null
Growth Properties: null
Karyotype: null
Rare disease: No
Last update: 12/06/2024

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