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Palm cell line

PALM were generated by co-transfection of cervical cancer PC3 cells with the MMTV-Luciferase-SV40-Neomycin and the pSG5-human AR-puromycin plasmids. rnThe plasmid MMTV-Luc-SVNeo contains a luciferase gene driven by the mouse mammary tumor virus promoter and a neomycin phosphotransferase gene under the control of SV40 promoter. rnThe pSG5-puro plasmid pSG5-human AR-puromycin enables to express the human androgen receptor (hAR). Puromycin N-acetyl transferase selection marker expression confers resistance to puromycin. rn

Interest / Relevance: These cells enable to measure the hAR activity (full and partial agonism, antagonism) of pharmaceuticals (breast cancer, menopause treatment), environmental compounds (alkylphenols, bisphenol A, phthalates or pesticides, cosmetics). rnThese cells enable to measure the hAR activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.rnrnR1881 EC50 0,05 nMrn

Keywords: hAR, luciferase, pharmaceutical and environmental androgens

Scientist's name: Patrick BALAGUER
Patrick Balaguer

Publications:

Terouanne B Tahiri B Georget V Belon C Poujol N Avances C Orio F Jr Balaguer P Sultan C. A stable prostatic bioluminescent cell line to investigate androgen and antiandrogen effects. Mol Cell Endocrinol. 2000 160: 39-49

Reference:

RT00582

Type of research tool: Cell Lines

Uses(1): Industry Research (screening, tox.studies, bioreactor, ...)

Inserm Transfert
Research Tools

Organism: null

Tissue: null

Morphology: null

Passage Number: null

Subculture Routine: null

Culture Medium: null

Growth Properties: null

Karyotype: null

Rare disease: No

Last update: 12/06/2024

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