Ms-5-omi/htra2-mcherry cells

Murine stromal MS-5 cells were transduced with a retroviral pBabe vector to stably express the Omi/HTRA2-mCherry protein. rnOMI/HTRA2 is a marker of intact polarized mitochondria that is rapidly degraded following mitochondrial outer membrane permeabilization (MOMP).

Interest / Relevance: Feeder cells for long-term culture of normal and Acute Leukemia Myeloid cellsrnmCherry fluorescence is a read-out of mitochondria integrity. rnMS5-Omi/HTRA2-mCherry cells can be used to study mitochondrial transfer.rnMouse Stromal cells
Keywords: Stromal cells , LTC-IC , Omi-mCherry , Mitochondrial transfer
Scientist's name: Véronique IMBERT
Jean-François PEYRON
Publications:
Moschoi R. Imbert V. Nebout M. Chiche J. Mary D. Prebet T. Saland E. Castellano R. Pouyet L. Collette Y. Vey N. Chabannon C. Recher C. Sarry J. E. Alcor D. Peyron J. F. and Griessinger E. (2016). Protective mitochondrial transfer from bone marrow stromal cells to acute myeloid leukemic cells during chemotherapy. Blood 128 253-264.

Reference:

RT00494

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Organism: Mouse
Tissue: Stromal cells
Morphology: Adherent cells
Passage Number: null
Subculture Routine: Split confluent culture 1:3 to 1:10 every third day using trypsin/EDTA
Culture Medium: IMDM supplemented with 2mM L-Glutamine 25mM Hepes 10%SVF Penicillin Streptomycin
Growth Properties: null
Karyotype: null
Rare disease: No
Last update: 12/06/2024

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