Mouse t lymphoma cell line – ko99l

The murine primary KO99L cell line was established by cultivating tumoral thymocytes from a tPTEN-/- mouse. Mice carrying a T cell-specific deletion of PTEN develop invasive T-cell lymphomas and die within 10-15 weeks. KO99L cells display an abnormal constitutive activation of the PI3K/Akt/mTOR pathway that supports their enhanced proliferation and deregulated metabolism, as well as their resistance to cell death. This cell line also displayed an amplification of c-Myc .

Interest / Relevance: Representative of T-cell Acute Lymphoblastic Leukemia/Lymphoblastic Lymphoma (T-ALL/LL) rnModel of mouse T lymphoma cells with constitutive activation of PI3/Akt/mTor et c-myc upregulation.rnIn vitro screening of antileukemics drugs-Combo experimentsrnTumorigenic in nude mice (subcutaneous injection).

Keywords: T-ALL/LL , PI3K/Akt/mTor , c-myc

Scientist's name: Véronique IMBERT
Jean-François PEYRON

Publications:

Rosilio C. Nebout M. Imbert V. Griessinger E. Neffati Z. Benadiba J. Hagenbeek T. Spits H. Reverso J. Ambrosetti D. Michiels J. F. Bailly-Maitre B. Endou H. Wempe M. F. and Peyron J. F. (2015). L-type amino-acid transporter 1 (LAT1): a therapeutic target supporting growth and survival of T-cell lymphoblastic lymphoma/T-cell acute lymphoblastic leukemia. Leukemia 29 1253-1266.rnrnrnRosilio C. Lounnas N. Nebout M. Imbert V. Hagenbeek T. Spits H. Asnafi V. Pontier-Bres R. Reverso J. Michiels J. F. Sahra I. B. Bost F. and Peyron J. F. (2013). The metabolic perturbators metformin phenformin and AICAR interfere with the growth and survival of murine PTEN-deficient T cell lymphomas and human T-ALL/T-LL cancer cells. Cancer Lett 336 114-126.rnrnrnLounnas N. Rosilio C. Nebout M. Mary D. Griessinger E. Neffati Z. Chiche J. Spits H. Hagenbeek T. J. Asnafi V. Poulsen S. A. Supuran C. T. Peyron J. F. and Imbert V. (2013). Pharmacological inhibition of carbonic anhydrase XII interferes with cell proliferation and induces cell apoptosis in T-cell lymphomas. Cancer Lett 333 76-88.

Reference:

RT00493