Mouse t lymphoma cell line expressing luciferase – ko99l/luc

The primary KO99L cell line was established by cultivating tumoral thymocytes from a tPTEN-/- mouse. KO99L cells were transduced with a pLenti-III-luc23 construct to stably express luciferase. rnSeveral clones are available.

Interest / Relevance: This cell line is suitale for in vitro and in vivo analyses of tumor growth by bioluminescence. rnTumorigenic in nude mice.
Keywords: T ALL/LL , Imaging , Tumor growth , Bioluminescence
Scientist's name: Véronique IMBERT
Jean-François PEYRON
Rosilio C. Lounnas N. Nebout M. Imbert V. Hagenbeek T. Spits H. Asnafi V. Pontier-Bres R. Reverso J. Michiels J. F. Sahra I. B. Bost F. and Peyron J. F. (2013). The metabolic perturbators metformin phenformin and AICAR interfere with the growth and survival of murine PTEN-deficient T cell lymphomas and human T-ALL/T-LL cancer cells. Cancer Lett 336 114-126.rnrnrnRosilio C. Nebout M. Imbert V. Griessinger E. Neffati Z. Benadiba J. Hagenbeek T. Spits H. Reverso J. Ambrosetti D. Michiels J. F. Bailly-Maitre B. Endou H. Wempe M. F. and Peyron J. F. (2015). L-type amino-acid transporter 1 (LAT1): a therapeutic target supporting growth and survival of T-cell lymphoblastic lymphoma/T-cell acute lymphoblastic leukemia. Leukemia 29 1253-1266.



Business Developper
Inserm Transfert
Research Tools
Organism: Mouse
Tissue: Thymocytes, T lymphoma
Morphology: Suspension, round cells
Passage Number: null
Subculture Routine: Medium Renewal: Every 2 to 3 days Dilution 1:10
Culture Medium: RPMI 1640 Glutamax supplemented with 20% FCS, 50 U/ml penicillin, 50 µg/ml streptomycin, and 1 mM sodium pyruvate.
Growth Properties: null
Karyotype: null
Rare disease: No
Last update: 12/06/2024

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