Method for universal detection and quantification of mycoplasma (mollicutes) 16s rdna by quantitative polymerase chain reaction amplifying a 1.5 kilobase fragment

The present invention relates to a method for detecting mycoplasma 16S rDNA 1.5 kilobase fragment amplified quantitative polymerase chain reaction (qPCR). The method according to the invention is suitable for the detection of potentially any Prokaryote contamination, especially mycoplasma contamination, in cell cultures. Another possible application can be its use in diagnosis and follow up of contamination in human, livestock, plants, food, or water for example.

Keywords: qPCR, mycoplasma
Patent Application number: EP 16 305 435.6 (Priority)
PCT/EP2017/058928
Inventors:
Audrey JEANBariza BLANQUIERIsabelle GROSJEANDenis GERLIER

Reference:

BIO15302-R1

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Patent filling date: 14-04-2016

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