Method for identifying effector treg cells for diagnosis, prognosis or treatment monitoring

Regulatory T (Treg) cells expressing the transcription factor FOXP3 play essential roles for the maintenance of immune self-tolerance and homeostasis, and in the suppression of effective immune responses against autologous cancer cells as well as invading microbes. But not all FOXP3-expressing CD4+ T cells in peripheral blood mononuclear cells (PBMCs) possess suppressive function and compose a heterogeneous population. It remains challenging, however, how these two populations with opposite functions, i.e., the highly suppressive FOXP3high eTreg population and the nonsuppressive and cytokine-secreting FOXP3low non-Treg population, can be more discretely and reliably differentiated by specific molecular markers, especially, those expressed on the cell surface. This is critically important, especially in preparing a pure suppression-competent Treg cell preparation for cell therapy of immunological diseases. The present invention relates to methods and kits for identifying effector regulatory T cells. In particular the present invention relates to use of CD15s as a biomarker for eTreg cells. The present invention also relates to a method for identifying effector Treg cells (eTreg) in a fluid sample comprising the steps of i) detecting the cell surface expression of CD4, CD25, CD127 and CD15s markers on the cell population contained in the fluid sample and ii) concluding that the cells expressing CD4, CD25, CD127 at low levels and CD15s are the effector Treg cells. The present invention also relates to a method for identifying effector Treg cells (eTreg) in a tissue sample comprising the steps of i) detecting the cell expression of CD4, CD25, Foxp3 and CD15s markers and ii) concluding that the cells expressing CD4, CD25, Foxp3 and CD15s are the effector Treg cells. Scientific publication(s): Proc Natl Acad Sci U S A., 2015 Jun 9, Miyara M. et al., Sialyl Lewis x (CD15s) identifies highly differentiated and most suppressive FOXP3high regulatory T cells in humans, doi: 10.1073/pnas.1508224112.

Keywords: Autoimmune diseases, Inflammatory diseases, Allergy, Graft Rejection, Cancer, Flow Cytometry, Immunoassay
Patent Application number: EP13 306 106.9
Inventors:
Guy GOROCHOVMakoto MIYARA
Publications:
Proc Natl Acad Sci U S A. 2015 Jun 9;112(23):7225-30. doi: 10.1073/pnas.1508224112. Epub 2015 May 26.

Reference:

BIO13081-D1

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Sylvestre CHEA
Sylvestre CHEA
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Patent filling date: 2013-07-31

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