This method for acquiring a spatial map of auditory perception of a subject comprises a plurality of successive test sequences, each test sequence comprising steps of:rnrna) calibration of the subject’s position, by displaying instructions to the subject, using a head-mounted visual display system worn by the subject, in order to acquire a reference position of the subject, the subject’s position being measured using a video motion capture system by measuring the spatial coordinates of a first optical marker worn by the subject,rnb) choosing spatial coordinates of a target location of a sound source, said target location being located around the subject, rnc) emitting a predefined sound, using a sound source placed at said target location,rnd) in response to acquisition instructions generated by the subject using an acquisition interface, acquiring an estimated location of said sound source, by using the video motion capture system to measure the spatial coordinates of a second optical marker held and pointed by the subject towards a perceived location of the sound source.