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Meln (her alpha) cell line

MELN were generated by transfection of breasr cancer MCF-7 cells with the ERE-betaGlobin-Luciferase-SV40-Neomycin plasmid. rnThe plasmid ERE-betaGlob-Luc-SVNeo contains a luciferase gene driven by estrogen receptor responsive element (ERE) in front of beta-globin promoter and a neomycin phosphotransferase gene under the control of SV40 promoter. rn

Interest / Relevance: These cells enable to measure the hER alpha estrogenic activity (full and partial agonism, antagonism) of pharmaceuticals (breast cancer, menopause treatment), environmental compounds (alkylphenols, bisphenol A, phthalates or pesticides, cosmetics). rnThese cells enable to measure the hER alpha estrogenic activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.rn

Keywords: Estrogen receptor alpha, luciferase, pharmaceutical and environmental estrogens

Scientist's name: Patrick BALAGUER
Patrick Balaguer

Publications:

Balaguer P Boussioux AM Demirpence E Nicolas JC. Reporter cell lines are useful tools for monitoring biological activity of nuclear receptor ligands. Luminescence. 2001 16:1 53-8. rnFenet H Gomez E Pillon A Rosain D Nicolas JC Casellas C Balaguer P. Estrogenic activity in water and sediments of a French river: contribution of alkylphenols. Arch Environ Contam Toxicol. 2003 44: 1-6. rn

Reference:

RT00583

Type of research tool: Cell Lines

Uses(1): Industry Research (screening, tox.studies, bioreactor, ...)

Inserm Transfert
Research Tools

Organism: null

Tissue: null

Morphology: null

Passage Number: null

Subculture Routine: null

Culture Medium: null

Growth Properties: null

Karyotype: null

Rare disease: No

Last update: 12/06/2024

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