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Hmln (hgr) reporter cell line

HMLN were generated by transfection of cervical cancer HeLa cells with the MMTV- -Luciferase-SV40-Neomycin plasmid. rnThe plasmid MMTV-Luc-SVNeo contains a luciferase gene driven by the mouse mammary tumor virus promoter and a neomycin phosphotransferase gene under the control of SV40 promoter. rn

Interest / Relevance: These cells enable to measure the hGR activity (full and partial agonism, antagonism) of pharmaceuticals (breast cancer, menopause treatment), environmental compounds (alkylphenols, bisphenol A, phthalates or pesticides, cosmetics). rnThese cells enable to measure the hGR activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.rn

Keywords: hGR, luciferase, pharmaceutical and environmental glucocorticoids

Scientist's name: Patrick BALAGUER
Patrick Balaguer

Publications:

null

Reference:

RT00622

Type of research tool: Cell Lines

Uses(1): Industry Research (screening, tox.studies, bioreactor, ...)

Inserm Transfert
Research Tools

Organism: null

Tissue: null

Morphology: null

Passage Number: null

Subculture Routine: null

Culture Medium: null

Growth Properties: null

Karyotype: null

Rare disease: No

Last update: 12/06/2024

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