Heln hrar alpha cell line

HELN were generated by transfection of cervical cancer HeLa cells with the ERE-betaGlobin-Luciferase-SV40-Neomycin plasmid.rnHELN hRAR alpha cells were generated by transfection of HELN cells with the pSG5-puro plasmid pSG5-human RAR alpha (ER alpha DBD)-puromycin.rnThe plasmid ERE-betaGlob-Luc-SVNeo contains a luciferase gene driven by estrogen receptor responsive element (ERE) in front of beta-globin promoter and a neomycin phosphotransferase gene under the control of SV40 promoter.rnThe pSG5-puro plasmid pSG5-human RAR alpha (ER alpha DBD)-puromycin enables to express the human retinoid receptor alpha in which the DNA binding domain (DBD) has been replaced by the estrogen receptor alpha DBD.rn

Interest / Relevance: These cells enable to measure the hRAR alpha activity (full and partial agonism, antagonism) of pharmaceuticals (breast cancer, menopause treatment), environmental compounds (alkylphenols, bisphenol A, phthalates or pesticides, cosmetics).rnThese cells enable to measure the hRAR alpha activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.rnrnTTNPB EC50 0.8 nM
Keywords: hRAR alpha, luciferase, natural, pharmaceutical and environmental retinoids
Scientist's name: Patrick BALAGUER
Patrick Balaguer
Publications:
Balaguer P Boussioux AM Demirpence E Nicolas JC. Reporter cell lines are useful tools for monitoring biological activity of nuclear receptor ligands. Luminescence. 2001 16:1 53-8

Reference:

RT00620

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Organism: null
Tissue: null
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Subculture Routine: null
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Growth Properties: null
Karyotype: null
Rare disease: No
Last update: 12/06/2024

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