Heln her alpha cell line

HELN were generated by transfection of cervical cancer HeLa cells with the ERE-betaGlobin-Luciferase-SV40-Neomycin plasmid. rnHELN hER alpha cells were generated by transfection of HELN cells with the plasmid pSG5-human ERalpha-puromycin. rn

Interest / Relevance: These cells enable to measure the hER alpha estrogenic activity (full and partial agonism, antagonism) of pharmaceuticals (breast cancer, menopause treatment), environmental compounds (alkylphenols, bisphenol A, phthalates or pesticides, cosmetics). rnThese cells enable to measure the hER alpha estrogenic activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.rnEstradiol EC50 17 pM

Keywords: hER alpha, luciferase, pharmaceutical and environmental estrogens

Scientist's name: Patrick BALAGUER
Patrick BALAGUER

Publications:

Escande A Pillon A Servant N Cravedi JP Larrea F Muhn P Nicolas JC Cavailles V Balaguer P. Evaluation of ligand selectivity using reporter cell lines stably expressing estrogen receptor alpha or beta. Biochem Pharmacol. 2006 71: 1459-69.rnDelfosse V Grimaldi M Pons JL Boulahtouf A le Maire A Cavailles V Labesse G Bourguet W Balaguer P. Structural and mechanistic insights into bisphenols action provide guidelines for risk assessment and discovery of bisphenol A substitutes. Proc Natl Acad Sci U S A. 2012 109 : 14930-5.rnDelfosse V Grimaldi M Cavaillès V Balaguer P Bourguet W. Structural and Functional Profiling of Estrogen Receptors Environmental Ligands. Environ Health Perspect. 2014 122: 1306-13.

Reference:

RT00316

Type of research tool: Cell Lines

Therapy area(1): Women health

Uses(1): Industry Research (screening, tox.studies, bioreactor, ...)

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Organism: human

Tissue: cervix

Morphology: null

Passage Number: null

Subculture Routine: null

Culture Medium: DMEM red phenol free, t 5% stripped serum , puromycine 0,5 microg/ml, geniticine 1 mg/ml

Growth Properties: null

Karyotype: null

Rare disease: No

Last update: 12/06/2024

Heln her alpha cell line

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