Back to results

Heln er alpha cell line

Cervical cancer cell line HeLa (ER negative) firstly transfected by a luciferase firefly gene under the control of an estrogen responsive element (ERE), secondly transfected by an ER alpha expression plasmid.
Interest / Relevance: These cells enable to measure the ER alpha estrogenic activity (full and partial agonism, antagonism) and the selectivity of pharmaceuticals (breast cancer, menopause treatment) or environmental compounds (industrials like alkylphenols, bisphenol A, phthalates or pesticides, cosmetics).
Estradiol EC50 (at this concentration, 50% of the maximal luciferase is obtained)is 16pM.
Scientist's name: Patrick BALAGUER

Reference:

RT00316

Business Developper
contact
Patrick BALAGUER
Inserm Transfert
Research Tools
Organism: human
Tissue: cervix
Culture Medium: DMEM red phenol free, t 5% stripped serum , puromycine 0,5 microg/ml, geniticine 1 mg/ml
Karyotype: ,estrogenic compound screening
Rare disease:
Last update: 18/11/2022

You might also be interested in

RT00412

Rat mesothelial cell line f2-3

Transformed rat mesothelial cell line isolated from the peritoneal fluid of a female rat (Fischer […]

Read more
RT00413

Rat mesothelial cell line f2-4

Transformed rat mesothelial cell line isolated from the peritoneal fluid of a female rat (Fischer […]

Read more
RT00425

Rat mesothelial cell line m2-0

Transformed rat mesothelial cell line isolated from the peritoneal fluid of a male rat (Fischer […]

Read more
RT00415

Rat mesothelial cell line f3-1

Transformed rat mesothelial cell line isolated from the peritoneal fluid of a female rat (Fischer […]

Read more
RT00427

Rat mesothelial cell line m2-3

Transformed rat mesothelial cell line isolated from the peritoneal fluid of a male rat (Fischer […]

Read more
RT00457

D3h2ln-at2 human invasive breast cancer cell line

D3H2LN-AT2 cell line stably expressing firefly luciferase, GFP and Flagged human AT2 receptor. […]

Read more
RT00430

Rat mesothelial cell line m2-1

Transformed rat mesothelial cell line isolated from the peritoneal fluid of a male rat (Fischer […]

Read more
RT00458

Rat transformed mesothelial cell line f5-3

Transformed rat mesothelial cell line (= myofibroblast) isolated from the peritoneal fibrosis […]

Read more
RT00492

Mouse t lymphoma cell line expressing luciferase

The primary KO99L cell line was established by cultivating tumoral thymocytes from a tPTEN-/- mouse. […]

Read more
RT00409

Rat mesothelial cell line f1-2

Transformed rat mesothelial cell line isolated from the peritoneal fluid of a female rat (Fischer […]

Read more
RT00431

Rat mesothelial cell line f5-2

Transformed rat mesothelial cell line isolated from the peritoneal fluid of a female rat (Fischer […]

Read more
RT00433

Conditional bioluminescent tmp3-alk cell line

Overexpression and activation of TMP3-ALK tyrosine kinase fusion protein is a causal oncogenic event […]

Read more