Cellular fibronectin toolset

Expression vectors encoding human cellular fibronectin variants harboring the alternatively spliced Extra Domains B and/or A (or neither) ; fibronectin variant-expressing cells and purified recombinant cellular fibronectin variant proteins.

Interest / Relevance: Cellular (also known as oncofetal) fibronectin variants harboring one or both alternatively spliced so-called extra domains (EDB and EDA) play a central bioregulatory role during development, repair processes and fibrosis.

Keywords: extracellular matrix, fibrosis, angiogenesis, tumor microenvironment

Scientist's name: Ellen VAN OBBERGHEN-SCHILLING
Georgios Efthymiou
Agata Radwanska

Publications:

Efthymiou G* Radwanska A* Grapa A-I Beghelli-de la Forest Divonne S Grall D Schaub S Hattab M Pisano S Poet M Pisani D Counillon L Descombes X Blanc-Feraud L and Van Obberghen-Schilling E. Fibronectin Extra Domains tune cellular responses and confer topographically distinct features to fibril networks. J Cell Sci. 2021 : jcs.252957 doi: 10.1242/jcs.252957 Published 1 February 2021.rnrnEfthymiou G* Saint A Ruff M Rekad Z Ciais D and Van Obberghen-Schilling E Shaping Up the Tumor Microenvironment with Cellular Fibronectin. Front Oncol. 2020 Apr 3010:641. doi: 10.3389/fonc.2020.00641.rnrnSoubies E Radwanska A Grall D Aubert GA Blanc-Feraud L Van Obberghen-Schilling E and Schaub S. Nanometric axial resolution of fibronectin assembly achieved with an efficient reconstruction approach for multi-angle-TIRF microscopy Sci Rep. 2019 Feb 139(1):1926. doi: 10.1038/s41598-018-36119-3.rnrnGrapa AI Meunier R Blanc-Féraud L Efthymiou G Schaub S Radwanska A Van Obberghen-Schilling E and Descombes X. (2018) Classification of fibronectin variants with curvelets IEEE International Symposium on Biomedical ImagingrnrnRadwanska A Grall D Schaub S Beghelli-de la Forest Divonne S Ciai D Sudaka A Rekima S Rupp T Orend G and Van Obberghen-Schilling E. (2017) Counterbalancing anti-adhesive effects of Tenascin-C through fibronectin expression in endothelial cells. Sci Rep. Oct 67(1):12762. doi: 10.1038/s41598-017-13008-9.rnrnGopal S* Veracini L* Grall D Butori C Schaub S Audebert S Camoin L Baudelet E Radwanska A Beghelli-de la Forest Divonne S Violette SM Weinreb PH Rekima S Ilie M Sudaka A Hofman P and Van Obberghen-Schilling E. Fibronectin-guided migration of carcinoma collectives. Nat Commun. 2017 Jan 198:14105. doi: 10.1038/ncomms14105rnrnSerres E. Debarbieux F.* Stanchi F.* Maggiorella L. Grall D. Rougon G. and Van Obberghen-Schilling E. (2014) Fibronectin expression in glioblastomas promotes cell cohesion collective invasion of basement membrane in vitro and orthotopic tumor growth in mice. Oncogene 33:3451-62.rnrnCseh B Fernandez-Sauze S Grall D Schaub S Doma E and Van Obberghen-Schilling E. Autocrine fibronectin directs matrix assembly and crosstalk between cell-matrix and cell-cell adhesion in vascular endothelial cells. J Cell Sci. (2010) 123 3989-3999.

Detailed technology overview: Detailed technology is described in : Efthymiou G, et al. Fibronectin Extra Domains tune cellular responses and confer topographically distinct features to fibril networks. J Cell Sci. 2021 : jcs.252957 doi: 10.1242/jcs.252957 Published 1 February 2021.- Construction of lentiviral expression vectors harboring the alternatively-spliced « cellular FN » variants containing Extra Domain A and/or B, or no Extra Domain (plasma-like FN)- Production of lentiviral particles for transduction of FN variant-expressing cells- Expression of FN variants in (assembly-incompetent) HEK293 cells- Purification of soluble FN variants secreted by FN-expressing HEK293 cells- Expression of FN variants in assembly-competent cells- Addition of purified FN variants to cells for functional studies- Preparation of variant-specific « cell-derived matrices »

Reference:

RT00508