Mouse t lymphoma cell line – ko99l

The murine primary KO99L cell line was established by cultivating tumoral thymocytes from a tPTEN-/- mouse. Mice carrying a T cell-specific deletion of PTEN develop invasive T-cell lymphomas and die within 10-15 weeks. KO99L cells display an abnormal constitutive activation of the PI3K/Akt/mTOR pathway that supports their enhanced proliferation and deregulated metabolism, as well as their resistance to cell death. This cell line also displayed an amplification of c-Myc .

Interest / Relevance: Representative of T-cell Acute Lymphoblastic Leukemia/Lymphoblastic Lymphoma (T-ALL/LL)
Model of mouse T lymphoma cells with constitutive activation of PI3/Akt/mTor et c-myc upregulation.
In vitro screening of antileukemics drugs-Combo experiments
Tumorigenic in nude mice (subcutaneous injection).
Keywords: T-ALL/LL , PI3K/Akt/mTor , c-myc
Scientist's name: Véronique IMBERT
Peyron Jean-Francois

Reference:

RT00493

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Véronique IMBERT
Inserm Transfert
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Organism: Mouse
Tissue: Thymocytes, T lymphoma
Morphology: Suspension, round cells
Subculture Routine: Medium Renewal: Every 2 to 3 days 1:10
Culture Medium: RPMI 1640 Glutamax supplemented with 20% FCS, 50 U/ml penicillin, 50 µg/ml streptomycin, and 1 mM sodium pyruvate.
Rare disease:
Last update: 15/12/2021

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