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Mouse t lymphoma cell line expressing luciferase – ko99l/luc

The primary KO99L cell line was established by cultivating tumoral thymocytes from a tPTEN-/- mouse. KO99L cells were transduced with a pLenti-III-luc23 construct to stably express luciferase. Several clones are available.

Interest / Relevance: This cell line is suitale for in vitro and in vivo analyses of tumor growth by bioluminescence.
Tumorigenic in nude mice.

Keywords: T ALL/LL , Imaging , Tumor growth , Bioluminescence

Scientist's name: Véronique IMBERT
Jean-Francois Peyron

Reference:

RT00492

Type of research tool: Cell Lines

Therapy area(1): Oncology

Therapy area(2): Others

Uses(1): Academic Research

Uses(2): Proof of concept in vitro

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Organism: Mouse

Tissue: Thymocytes, T lymphoma

Morphology: Suspension, round cells

Subculture Routine: Medium Renewal: Every 2 to 3 days Dilution 1:10

Culture Medium: RPMI 1640 Glutamax supplemented with 20% FCS, 50 U/ml penicillin, 50 µg/ml streptomycin, and 1 mM sodium pyruvate.

Rare disease:

Last update: 13/01/2022

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