Hg5ln rtr alpha cell line

HG5LN were generated by transfection of cervical cancer HeLa cells with the (GAL4RE)5-betaGlobin-Luciferase-SV40-Neomycin plasmid. rnHG5LN GAL4-rTRalpha cells were generated by transfection of HG5LN cells with the plasmid pSG5-GAL4-rat TR alpha-puromycin. rnrnThe plasmid (GAL4RE)5-betaGlob-Luc-SVNeo contains a luciferase gene driven by a pentamer of yeast activator GAL4 binding sites in front of beta-globin promoter and a neomycin phosphotransferase gene under the control of SV40 promoter. rnThe pSG5-puro plasmid pSG5-GAL4-rTR alpha-puromycin enables to express the DNA binding domain of the yeast activator GAL4 (GAL4 DBD) followed by the ligand binding domain of the rat thyroid receptor alpha (rTR alpha LBD). Puromycin N-acetyl transferase selection marker expression confers resistance to puromycin.rnrn

Interest / Relevance: These cells enable to measure the rTR alpha (full and partial agonism, antagonism) of pharmaceuticals, environmental compoundsrnThese cells enable to measure the rTR alpha activity of environmental (water, sediments), human (blood, urine adipose tissue) or food (water, vegetables, meat) samples.rnT3 EC50 0.8 nMrn
Keywords: TR alpha, luciferase, pharmaceutical and environmental chemicals
Scientist's name: Patrick BALAGUER
Patrick Balaguer
Publications:
Fini JB Riu A Debrauwer L Hillenweck A Mével SL Chevolleau S Boulahtouf A PalmierK Balaguer P Cravedi JP Demeneix BA Zalko D. Parallel biotransformation of Tetrabromobisphenol A in Xenopus laevis and mammals: Xenopus as a model for endocrine perturbation studies. Toxicol Sci. 2012 125 : 359-67.

Reference:

RT00585

Business Developper
contact
Inserm Transfert
Research Tools
Organism: null
Tissue: null
Morphology: null
Passage Number: null
Subculture Routine: null
Culture Medium: null
Growth Properties: null
Karyotype: null
Rare disease: No
Last update: 12/06/2024

You might also be interested in